Selective UV Crosslinking of Peptides and Functional Antibodies

Tech ID: 12-025

Inventors: Nathan J. Alves, Zihni Basar Bilgicer,


A gentle method for covalently attaching peptides and small molecules to antibodies.

Technology Summary

Current immunosensors often rely on the random orientation of antibodies nonspecifically adsorbed to surfaces which renders up to 90% of antibodies inactive through sterically hindering access to their binding sites, or negatively impacting antibody conformation.

Researchers at the University of Notre Dame have developed a novel method that results in a unique and consistent covalent insertion of a target molecule selectively to the antibody light chains without disrupting the antibodies’ ability to bind its respective antigen nor impact Fc recognition. Using this method, an immunosensor has been developed that maintains nearly 100% antibody activity through the oriented immobilization of antibodies onto an ELISA surface.  Validation of this initial proof of concept demonstrates the potential for this process to enhance the detection abilities of many currently employed immunosensor technologies, as well as detection modalities currently being developed for lab-on-a-chip applications.  This method of crosslinking also has implications in the development of next generation pharmaceuticals such as: chemotherapeutics, cell penetrating peptides, targeting sequences, imaging specific molecules, and facilitating oriented immobilization of antibodies onto several nanoparticle drug delivery platforms such as liposomes. 

Market Advantages

  • Maintains nearly 100% antibody activity
  • Enhances the detection abilities of immunosensor technologies and 2nd generation antibody-based pharmaceuticals

Technology Readiness Level

TRL 4 - Lab Validation

Intellectual Property

US serial no. 16/450,615, EPO pat. no. 2970440



Richard Cox