Factor XII Deficient Mice

Tech ID: 13-024

Inventors: Dr. Francis Castellino

Date Added: August 22, 2019

 

Overview

Genetically-transmissible factor XII-deficient mice for use in lab settings.

Technology Summary

13 024

A genetically-transmissible factor (F) XII-inactivated allele has been produced in mice by targeted replacement of exons 3-8 of the FXII gene with the neomycin resistance gene.  Interbreeding of these mice provided offspring homozygous for two inactivated FXII alleles (FXII-/-). Male and female FXII-deficient mice bred normally in all genotypic combinations of the heterozygous and homozygous states, and the offspring survived to adulthood, suggesting that a total FXII deficiency does not affect embryonic development and survival. Neither FXII transcripts nor FXII antigen was found in various tissues of adult FXII-/- mice. No obvious unchallenged coagulopathies were present in FXII-/- adult mice, despite greatly prolonged activated partial thromboplastin times in this mouse cohort.

Technology Readiness Status

TRL 9 - Previous Successful Application 

Publication

Plasma Levels of Bradykinin are Suppressed in Factor XII-Deficient Mice
DOI: 10.1160/TH06-03-0128

Contact

Richard Cox

rcox4@nd.edu

574.631.5158